ENZ / Enzo Biochem, Inc. - Depositi SEC, Relazione annuale, dichiarazione di delega

Enzo Biochem, Inc.
US ˙ OTCPK ˙ US2941001024
QUESTO SIMBOLO NON E' PIU' ATTIVO

Statistiche di base
LEI 5299001N23WBO4XQ2P60
CIK 316253
SEC Filings
All companies that sell securities in the United States must register with the Securities and Exchange Commission (SEC) and file reports on a regular basis. These reports include company annual reports (10K, 10Q), news updates (8K), investor presentations (found in 8Ks), insider trades (form 4), ownership reports (13D, and 13G), and reports related to the specific securities sold, such as registration statements and prospectus. This page shows recent SEC filings related to Enzo Biochem, Inc.
SEC Filings (Chronological Order)
Questa pagina fornisce un elenco completo e cronologico dei depositi SEC, esclusi i documenti di proprietà che forniamo altrove.
August 20, 2025 POS AM

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 333-271911 No. 333-272727 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 1 to FORM S-3 REGISTRATION STATEMENT No. 333-271911 Post-Effective Amendment No. 1 to FORM S-3 REGISTRATION STATEMENT No. 333-272727 UNDER THE SECURITIES ACT OF 1933 ENZO BIOCHEM, INC. (Exact name

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 EX-3.1

FORM OF CERTIFICATE OF AMENDMENT OF THE CERTIFICATE OF INCORPORATION OF ENZO BIOCHEM, INC. ARTICLE I

Exhibit 3.1 FORM OF CERTIFICATE OF AMENDMENT OF THE CERTIFICATE OF INCORPORATION OF ENZO BIOCHEM, INC. ARTICLE I The name of the corporation is “Enzo Biochem, Inc.” (the “Corporation”). ARTICLE II The purpose for which the Corporation is formed is to engage in any lawful act or activity for which corporations may be organized under the New York Business Corporation Law, provided that the Corporati

August 20, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): August 20, 2025 ENZO BIOCHEM, INC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): August 20, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 EX-3.2

AMENDED AND RESTATED BY-LAWS OF ENZO BIOCHEM, INC. ARTICLE I OFFICES

Exhibit 3.2 AMENDED AND RESTATED BY-LAWS OF ENZO BIOCHEM, INC. ARTICLE I OFFICES Section 1.01 Principal Office. The principal office of the corporation shall be located at such place within or without the State of New York as the board of directors may from time to time determine. Section 1.02 Other Offices. The corporation may also have offices and places of business at such other places within a

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 POS AM

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 333-271911 No. 333-272727 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 1 to FORM S-3 REGISTRATION STATEMENT No. 333-271911 Post-Effective Amendment No. 1 to FORM S-3 REGISTRATION STATEMENT No. 333-272727 UNDER THE SECURITIES ACT OF 1933 ENZO BIOCHEM, INC. (Exact name

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 S-8 POS

As filed with the Securities and Exchange Commission on August 20, 2025

As filed with the Securities and Exchange Commission on August 20, 2025 No. 33-88826 No. 333-87153 No. 333-89308 No. 333-123712 No. 333-172127 No. 333-197028 No. 333-226799 No. 333-236958 No. 333-252159 No. 333-260894 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Post-Effective Amendment No. 2 to FORM S-8 REGISTRATION STATEMENT No. 33-88826 Post-Effective Amendment No. 1

August 20, 2025 15-12G

FORM 15 CERTIFICATION AND NOTICE OF TERMINATION OF REGISTRATION UNDER SECTION 12(g) OF THE SECURITIES EXCHANGE ACT OF 1934 OR SUSPENSION OF DUTY TO FILE REPORTS UNDER SECTIONS 13 AND 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934.

OMB APPROVAL UNITED STATES OMB Number: 3235-0167 SECURITIES AND EXCHANGE COMMISSION Expires: August 31, 2027 Washington, D.

August 19, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): August 19, 2025 ENZO BIOCHEM, INC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): August 19, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

August 8, 2025 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☐ Filed by a Party other than the Registrant ☒ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (a

July 22, 2025 DEFM14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Defin

July 7, 2025 EX-FILING FEES

Calculation of Filing Fee Tables Schedule 14A (Form Type) Enzo Biochem, Inc. (Exact Name of Registrant as Specified in its Charter) Table 1: Transaction Valuation

Exhibit 107 Calculation of Filing Fee Tables Schedule 14A (Form Type) Enzo Biochem, Inc.

July 7, 2025 PREM14A

PRELIMINARY PROXY STATEMENT — SUBJECT TO COMPLETION, DATED JULY 3, 2025 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment N

PRELIMINARY PROXY STATEMENT — SUBJECT TO COMPLETION, DATED JULY 3, 2025 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.

June 30, 2025 11-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ☒ ANNUAL REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ☒ ANNUAL REPORT PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 FOR THE FISCAL YEAR ENDED DECEMBER 31, 2024 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 1

June 24, 2025 EX-10.1

warrant Cancellation Agreement

Exhibit 10.1 warrant Cancellation Agreement This WARRANT Cancellation Agreement (this “Agreement”) is made and entered into as of this , 2025, by and between (the “Warrantholder”), Enzo Biochem, Inc., a New York corporation (the “Company”) in contemplation of a Fundamental Transaction. Capitalized terms used herein and not otherwise defined shall have the meanings given to such terms in the Common

June 24, 2025 EX-2.1

Agreement and Plan of merger by and among Bethpage Parent, Inc., Bethpage Merger Sub, Inc. Enzo Biochem, Inc. Dated as of June 23, 2025 Table of Contents

Exhibit 2.1 Agreement and Plan of merger by and among Bethpage Parent, Inc., Bethpage Merger Sub, Inc. and Enzo Biochem, Inc. Dated as of June 23, 2025 Table of Contents Page ARTICLE 1 THE MERGER 2 Section 1.1 The Merger 2 Section 1.2 Effective Time of Merger 2 Section 1.3 General Effects of Merger 2 Section 1.4 Effect of Merger on Capital Stock 2 Section 1.5 Treatment of Company Options, Company

June 24, 2025 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☐ Filed by a Party other than the Registrant ☒ Check the appropriate box: ☐ Preliminary Consent Statement ☐ Confidential, for Use of the Commission Only

June 24, 2025 EX-99.1

Enzo Biochem, Inc. To be Acquired by Battery Ventures in All-Cash Transaction Transaction Follows Completion of Comprehensive Strategic Review by Special Committee of the Board to Maximize Shareholder Value

Exhibit 99.1 Enzo Biochem, Inc. To be Acquired by Battery Ventures in All-Cash Transaction Transaction Follows Completion of Comprehensive Strategic Review by Special Committee of the Board to Maximize Shareholder Value Farmingdale, NY, June 24, 2025 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (OTCQX: ENZB) (“Enzo” or the “Company”) today announced that on June 23, 2025, following the market close, it e

June 24, 2025 EX-99.1

Enzo Biochem, Inc. To be Acquired by Battery Ventures in All-Cash Transaction Transaction Follows Completion of Comprehensive Strategic Review by Special Committee of the Board to Maximize Shareholder Value

Exhibit 99.1 Enzo Biochem, Inc. To be Acquired by Battery Ventures in All-Cash Transaction Transaction Follows Completion of Comprehensive Strategic Review by Special Committee of the Board to Maximize Shareholder Value Farmingdale, NY, June 24, 2025 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (OTCQX: ENZB) (“Enzo” or the “Company”) today announced that on June 23, 2025, following the market close, it e

June 24, 2025 EX-2.1

Agreement and Plan of merger by and among Bethpage Parent, Inc., Bethpage Merger Sub, Inc. Enzo Biochem, Inc. Dated as of June 23, 2025 Table of Contents

Exhibit 2.1 Agreement and Plan of merger by and among Bethpage Parent, Inc., Bethpage Merger Sub, Inc. and Enzo Biochem, Inc. Dated as of June 23, 2025 Table of Contents Page ARTICLE 1 THE MERGER 2 Section 1.1 The Merger 2 Section 1.2 Effective Time of Merger 2 Section 1.3 General Effects of Merger 2 Section 1.4 Effect of Merger on Capital Stock 2 Section 1.5 Treatment of Company Options, Company

June 24, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): June 24, 2025 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): June 24, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File N

June 24, 2025 EX-10.2

VOTING AND Support AGREEMENT

Exhibit 10.2 VOTING AND Support AGREEMENT This VOTING AND SUPPORT AGREEMENT (this “Agreement”) is made and entered into as of [●], 2025, by and among [●] Parent, Inc., a Delaware corporation (“Parent”), [●] Merger Sub, Inc., a New York corporation and a wholly owned subsidiary of Parent (“Merger Sub”), and the stockholders of Enzo Biochem, Inc., a New York corporation (the “Company”) listed on Sch

June 24, 2025 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): June 24, 2025 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): June 24, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File N

June 24, 2025 EX-10.1

warrant Cancellation Agreement

Exhibit 10.1 warrant Cancellation Agreement This WARRANT Cancellation Agreement (this “Agreement”) is made and entered into as of this , 2025, by and between (the “Warrantholder”), Enzo Biochem, Inc., a New York corporation (the “Company”) in contemplation of a Fundamental Transaction. Capitalized terms used herein and not otherwise defined shall have the meanings given to such terms in the Common

June 24, 2025 EX-10.2

VOTING AND Support AGREEMENT

Exhibit 10.2 VOTING AND Support AGREEMENT This VOTING AND SUPPORT AGREEMENT (this “Agreement”) is made and entered into as of [●], 2025, by and among [●] Parent, Inc., a Delaware corporation (“Parent”), [●] Merger Sub, Inc., a New York corporation and a wholly owned subsidiary of Parent (“Merger Sub”), and the stockholders of Enzo Biochem, Inc., a New York corporation (the “Company”) listed on Sch

June 16, 2025 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2025 ☐ TRANSITION REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2025 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM

April 22, 2025 EX-99.1

Enzo Biochem, Inc. Announces Review of Strategic Alternatives after Receiving Multiple Inquiries Regarding a Potential Transaction Forms Special Committee of the Board to Lead Evaluation of Viable Options for Maximizing Shareholder Value Engages Inde

Exhibit 99.1 Enzo Biochem, Inc. Announces Review of Strategic Alternatives after Receiving Multiple Inquiries Regarding a Potential Transaction Forms Special Committee of the Board to Lead Evaluation of Viable Options for Maximizing Shareholder Value Engages Independent Financial and Legal Advisors to Support Thorough Process Remains Committed to Maintaining Focus on Cost Containment, Cash Preserv

April 22, 2025 8-K

Financial Statements and Exhibits, Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): April 22, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

April 21, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): April 21, 2025 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): April 21, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

April 7, 2025 25

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 25 NOTIFICATION OF REMOVAL FROM LISTING AND/OR REGISTRATION UNDER SECTION 12(B) OF THE SECURITIES EXCHANGE ACT OF 1934 Commission File Number 001-09974 ENZO BIOCHEM, INC. TH

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 25 NOTIFICATION OF REMOVAL FROM LISTING AND/OR REGISTRATION UNDER SECTION 12(B) OF THE SECURITIES EXCHANGE ACT OF 1934 Commission File Number 001-09974 ENZO BIOCHEM, INC. THE NEW YORK STOCK EXCHANGE (Exact name of Issuer as specified in its charter, and name of Exchange where security is listed and/or registered) 21 Execu

March 31, 2025 EX-99.1

Enzo Biochem Announces Notification to New York Stock Exchange of Intention to Voluntarily Delist Common Stock and Transfer Trading of Shares to OTCQX

Exhibit 99.1 Enzo Biochem Announces Notification to New York Stock Exchange of Intention to Voluntarily Delist Common Stock and Transfer Trading of Shares to OTCQX FARMINGDALE, N.Y., March 28, 2025 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (“Enzo” or the “Company”) today announced its intention to voluntarily delist its common stock from The New York Stock Exchange (“NYSE”) and to have its

March 31, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): March 28, 2025 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): March 28, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

March 18, 2025 EX-99.1

Enzo Biochem Reports Second Quarter Fiscal Year 2025 Results

Exhibit 99.1 Enzo Biochem Reports Second Quarter Fiscal Year 2025 Results FARMINGDALE, N.Y., March 17, 2025 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (“Enzo” or the “Company”) today announced financial results for the fiscal second quarter ended January 31, 2025, with sequential quarter improvement in revenue, gross margin and operating profit / loss. Financial Highlights ● The Company’s s

March 18, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): March 17, 2025 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): March 17, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

March 17, 2025 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2025 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2025 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

January 16, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 15, 2025 ENZO BIOCHEM, IN

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 15, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission Fil

January 15, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 13, 2025 ENZO BIOCHEM, IN

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 13, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission Fil

January 14, 2025 EX-99.1

Enzo Biochem Receives Notice of Non-Compliance with New York Stock Exchange Listing Rules

Exhibit 99.1 Enzo Biochem Receives Notice of Non-Compliance with New York Stock Exchange Listing Rules FARMINGDALE, N.Y., January 14, 2025 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (“Enzo” or the “Company”) today announced that it was notified by the New York Stock Exchange (the “NYSE”) that it is not in compliance with Section 802.01B and Section 802.01C of the NYSE Listed Company Manual

January 14, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 8, 2025 ENZO BIOCHEM, INC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 8, 2025 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

December 16, 2024 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2024 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2024 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

November 27, 2024 DEF 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Definitive Proxy State

November 25, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): November 22, 2024 ENZO BIOCHEM, I

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): November 22, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission Fi

October 30, 2024 EX-99.1

ENZO BIOCHEM, INC. (in thousands, except per share data)

Exhibit 99.1 Enzo Biochem Reports Fourth Quarter and Fiscal Year 2024 Results and Announces Cash Dividend FARMINGDALE, N.Y., Oct. 29, 2024 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (“Enzo” or the “Company”) today announced financial results for the fiscal fourth quarter ended July 31, 2024 and the fiscal year ended July 31, 2024. The Company also announced that its board of directors had a

October 30, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): October 29, 2024 ENZO BIOCHEM, IN

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): October 29, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission Fil

October 29, 2024 EX-31.B

Certification of CFO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

Exhibit 31 (b) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

October 29, 2024 EX-31.A

Certification of CEO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

Exhibit 31 (a) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

October 29, 2024 EX-32.B

Certification of CFO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

Exhibit 32 (b) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2024 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Patricia Eckert, Chief Financia

October 29, 2024 EX-32.A

Certification of CEO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

Exhibit 32 (a) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2024 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Kara Cannon, Chief Executive Of

October 29, 2024 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2024 ☐ TRANSITION REPORT PURSUANT TO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2024 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM, INC.

October 29, 2024 EX-4.1

Description of Capital Stock

Exhibit 4.1 DESCRIPTION OF CAPITAL STOCK General Matters Pursuant to our Certificate of Incorporation, as amended, the total amount of our authorized capital stock is 100,000,000 shares, which consists of 75,000,000 shares of authorized common stock, par value $0.01 per share, and 25,000,000 shares of authorized preferred stock, par value $0.01 per share. As of October 25, 2024, we had outstanding

October 29, 2024 EX-21

List of subsidiaries of the Company

Exhibit 21 List of subsidiaries of the Company Enzo Clinical Labs, Inc., a New York Corporation Enzo Life Sciences, Inc., a New York Corporation Enzo Life Sciences (ELS) AG, in Lausen, Switzerland, a wholly-owned subsidiary of Enzo Life Sciences, Inc. Enzo Therapeutics, Inc., a New York Corporation Enzo Realty LLC, a New York Corporation Enzo Realty II, LLC, a New York Corporation

September 24, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): September 20, 2024 ENZO BIOCHEM,

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): September 20, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission F

August 14, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): August 13, 2024 ENZO BIOCHEM, INC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): August 13, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

August 2, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): July 29, 2024 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): July 29, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File N

July 25, 2024 EX-10.1

Executive Employment Agreement between Enzo Biochem, Inc. and Patricia Eckert, effective as of June 3, 2024

Exhibit 10.1 EXECUTIVE EMPLOYMENT AGREEMENT This Executive Employment Agreement (the “Agreement”), dated as of June 03, 2024 (the “Effective Date”), is made between Enzo Biochem, Inc., a New York corporation, with its principal office at 21 Executive Boulevard, Farmingdale, New York 11735 (the “Company”) and Patricia Eckert (the “Executive”) (each individually a “Party”, and collectively the “Part

July 25, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): July 24, 2024 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): July 24, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File N

June 26, 2024 11-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ☒ ANNUAL REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ☒ ANNUAL REPORT PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 FOR THE FISCAL YEAR ENDED DECEMBER 31, 2023 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 1

June 13, 2024 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2024 ☐ TRANSITION REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2024 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM

May 24, 2024 EX-10.1

Second Amended and Restated Executive Employment Agreement between Enzo Biochem, Inc. and Kara Cannon, effective as of January 1, 2024

Exhibit 10.1 SECOND AMENDED AND RESTATED EXECUTIVE EMPLOYMENT AGREEMENT This Second Amended and Restated Executive Employment Agreement (the “Agreement”), dated as of January 1, 2024 (the “Effective Date”), is made between Enzo Biochem, Inc., a New York corporation, with its principal office at 81 Executive Boulevard, Farmingdale, New York 11735 (the “Company”) and Kara Cannon (the “Executive”) (e

May 24, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): May 23, 2024 ENZO BIOCHEM, INC. (

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): May 23, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File Nu

March 13, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): March 13, 2024 ENZO BIOCHEM, INC.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): March 13, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission File

March 13, 2024 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2024 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2024 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

March 13, 2024 EX-99.1

ENZO BIOCHEM, INC. (in thousands, except per share data)

Exhibit 99.1 Enzo Biochem Reports Second Quarter Fiscal 2024 Results and Provides Business Update FARMINGDALE, N.Y., March 13, 2024 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (“Enzo” or the “Company”) today announced financial results for the second quarter ended January 31, 2024. Second Quarter Highlights ● The Company's Life Science division's second-quarter revenue of $8.5 million improv

February 27, 2024 8-K

Departure of Directors or Certain Officers; Election of Directors; Appointment of Certain Officers; Compensatory Arrangements of Certain Officers

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): February 23, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission Fi

February 14, 2024 SC 13G/A

ENZ / Enzo Biochem, Inc. / 1 MAIN CAPITAL MANAGEMENT, LLC - SC 13G/A Passive Investment

SC 13G/A 1 tm245967d1sc13ga.htm SC 13G/A UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G Under the Securities Exchange Act of 1934 (Amendment No. 1)* Enzo Biochem, Inc. (Name of Issuer) Common Stock, $0.01 par value per share (Title of Class of Securities) 294100102 (CUSIP Number) December 31, 2023 (Date of Event Which Requires Filing of this statement) Check t

February 1, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 31, 2024 ENZO BIOCHEM, IN

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): January 31, 2024 ENZO BIOCHEM, INC. (Exact name of registrant as specified in its charter) New York 001-09974 13-2866202 (State or other jurisdiction of incorporation) (Commission Fil

February 1, 2024 EX-99.1

Enzo Biochem Reports Results of its Annual Meeting of Shareholders and Officer Appointments

Exhibit 99.1 Enzo Biochem Reports Results of its Annual Meeting of Shareholders and Officer Appointments FARMINGDALE, NY, Feb 1, 2024 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (“Enzo” or the “Company”), a leading life science company, announced the voting results from its Annual Meeting held on January 31, 2024 and officer appointments. Summary of the Fiscal Year 2023 Annual Shareholder Me

December 21, 2023 DEFR14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. 1)

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. 1) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Defi

December 15, 2023 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2023 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2023 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

November 28, 2023 10-K/A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 1) (Mark One) ☒ Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2023 ☐ Transiti

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 1) (Mark One) ☒ Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2023 or ☐ Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the transition period from to Commission File Number: 001-0997

November 3, 2023 EX-31.A

Certification of CEO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

EXHIBIT 31 (a) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

November 3, 2023 EX-10.X

Separation Agreement and General Release between Hamid Erfanian and Enzo Biochem, Inc.

EXHIBIT 10 (x) EXECUTION COPY SEPARATION AGREEMENT AND GENERAL RELEASE This Separation Agreement and General Release (“Agreement”) by and between Hamid Erfanian (“Executive” or “you”) and Enzo Biochem, Inc.

November 3, 2023 EX-10.W

Amendment Agreement by and among JGB Capital, LP, JGB (Cayman) Sussex Ltd., Enzo Biochem, Inc. and JGB Collateral LLC

EXHIBIT 10 (w) AMENDMENT AGREEMENT This Amendment Agreement (“Agreement”), dated as of October 4, 2023, is made by and between JGB Capital, LP, JGB Partners, LP and JGB (Cayman) Sussex Ltd.

November 3, 2023 EX-32.B

Certification of CFO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

EXHIBIT 32 (b) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2023 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Patricia Eckert, Interim Chief

November 3, 2023 EX-10.Y

Settlement Agreement between Barry Weiner, Shahla Weiner, Roya Weiner, and Jonathan Weiner and Enzo Biochem, Inc., Mary Tagliaferri, Ian Walters, Brad Radoff, and Hamid Erfanian

Exhibit 10 (y) SETTLEMENT AGREEMENT This Settlement Agreement, dated as of October 24, 2023 ("Agreement"), is entered into by and between Barry Weiner ("Weiner"), Shahla Weiner, Roya Weiner, and Jonathan Weiner (collectively, the foregoing are the "Weiner Parties" and each is a "Weiner Party"), Enzo Biochem, Inc.

November 3, 2023 EX-31.B

Certification of CFO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

EXHIBIT 31 (b) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

November 3, 2023 EX-32.A

Certification of CEO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

EXHIBIT 32 (a) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2023 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Kara Cannon, Interim Chief Exec

November 3, 2023 EX-97.1

Clawback Policy

EXHIBIT 97.1 ENZO BIOCHEM, INC. (the “Company”) Compensation Clawback Policy Effective Date: October 1, 2023 1. Purpose. The Company has adopted this Policy to comply with Section 954 of the Dodd-Frank Wall Street Reform and Consumer Protection Act of 2010, as codified by Section 10D of the Exchange Act, and Section 303A.14 of the NYSE Listed Company Manual, which require the recovery of certain f

November 3, 2023 EX-4.1

Description of Capital Stock

Exhibit 4.1 DESCRIPTION OF CAPITAL STOCK General Matters Pursuant to our Certificate of Incorporation, as amended, the total amount of our authorized capital stock is 100,000,000 shares, which consists of 75,000,000 shares of authorized common stock, par value $0.01 per share, and 25,000,000 shares of authorized preferred stock, par value $0.01 per share. As of October 27, 2023, we had outstanding

November 3, 2023 EX-21

List of subsidiaries of the Company

EXHIBIT 21 List of subsidiaries of the Company Enzo Clinical Labs, Inc., a New York Corporation Enzo Life Sciences, Inc., a New York Corporation Enzo Life Sciences (ELS) AG, in Lausen, Switzerland, a wholly-owned subsidiary of Enzo Life Sciences, Inc. Enzo Therapeutics, Inc., a New York Corporation Enzo Realty LLC, a New York Corporation Enzo Realty II, LLC, a New York Corporation

November 3, 2023 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2023 ☐ TRANSITION REPORT PURSUANT TO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2023 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM, INC.

October 31, 2023 NT 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 12b-25 NOTIFICATION OF LATE FILING

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 12b-25 NOTIFICATION OF LATE FILING OMB APPROVAL OMB Number: 3235-0058 Estimated average burden hours per response ... 2.50 SEC FILE NUMBER 001-09974 CUSIP NUMBER 294100102 (Check one): ☒ Form 10-K ☐ Form 20-F ☐ Form 11-K ☐ Form 10-Q ☐ Form 10-D ☐ Form N-CEN ☐ Form N-CSR For Period Ended: July 31, 2023 ☐ Transition Report

October 30, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): October 26, 2023 Enzo Biochem, Inc.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): October 26, 2023 Enzo Biochem, Inc.

October 30, 2023 EX-3.1

Amended and Restated Bylaws (15)

Exhibit 3.1 AMENDED AND RESTATED BY-LAWS OF ENZO BIOCHEM, INC. (a New York Corporation) (as amended and restated as of October 26, 2023) ARTICLE I MEETING OF SHAREHOLDERS Section 1. Annual Meeting. The annual meeting of the shareholders of Enzo Biochem, Inc., (hereinafter called the “Corporation”) for the election of directors and for the transaction of such other business as may come before the m

September 11, 2023 SC 13G

ENZ / Enzo Biochem, Inc. / 1 MAIN CAPITAL MANAGEMENT, LLC - SC 13G Passive Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G Under the Securities Exchange Act of 1934 (Amendment No. )* Enzo Biochem, Inc. (Name of Issuer) Common Stock, $0.01 par value per share (Title of Class of Securities) 294100102 (CUSIP Number) September 1, 2023 (Date of Event Which Requires Filing of this statement) Check the appropriate box to designate the rule p

September 11, 2023 EX-99.1

Joint Filing Agreement

EX-99.1 2 tm2325875d1ex-1.htm EXHIBIT 1 Exhibit 1 JOINT FILING AGREEMENT In accordance with Rule 13d-1(k) under the Securities Exchange Act of 1934, as amended, the persons named below agree to the joint filing on behalf of each of them of a statement on Schedule 13G (including amendments thereto) with respect to the Common Stock, $0.01 par value per share, of Enzo Biochem, Inc., and further agree

September 7, 2023 EX-99.1

Enzo Biochem Announces Departure of Hamid Erfanian and Appointment of Kara Cannon as Interim CEO

Exhibit 99.1 Enzo Biochem Announces Departure of Hamid Erfanian and Appointment of Kara Cannon as Interim CEO FARMINGDALE, NY, Sept. 06, 2023 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) announced today that Hamid Erfanian, Chief Executive Officer (CEO), will be resigning his seat on the Board of Directors and departing the Company effective September 5, 2023, with Enzo’s Chief Operating Offi

September 7, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): September 5, 2023 Enzo Biochem, Inc

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): September 5, 2023 Enzo Biochem, Inc.

July 28, 2023 EX-99.1

UNAUDITED PRO FORMA CONDENSED CONSOLIDATED FINANCIAL INFORMATION (in thousands, except share and per share data)

Exhibit 99.1 UNAUDITED PRO FORMA CONDENSED CONSOLIDATED FINANCIAL INFORMATION (in thousands, except share and per share data) The following unaudited pro forma condensed consolidated financial information is based upon the historical consolidated statements of Enzo Biochem, Inc., a New York corporation (the “Company”), adjusted to give effect to the sale (the “Asset Sale”) of substantially all the

July 28, 2023 8-K/A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K/A (Amendment No. 1) CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): July 24, 2023 E

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K/A (Amendment No.

July 24, 2023 EX-99.1

Enzo Biochem Completes Sale of Clinical Laboratory to Labcorp Significant value unlocked for Enzo shareholders with continued focus on life sciences.

Exhibit 99.1 Enzo Biochem Completes Sale of Clinical Laboratory to Labcorp Significant value unlocked for Enzo shareholders with continued focus on life sciences. FARMINGDALE, NY, July 24, 2023 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE:ENZ) today announced that it has closed a transaction to sell certain assets of Enzo’s Clinical laboratory division (Enzo Clinical Labs) to Laboratory Corporation

July 24, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): July 24, 2023 Enzo Biochem, Inc. (E

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): July 24, 2023 Enzo Biochem, Inc.

July 24, 2023 SC 13D/A

ENZ / Enzo Biochem, Inc. / Laboratory Corp Of America Holdings - SC 13D/A Activist Investment

SC 13D/A SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D UNDER THE SECURITIES EXCHANGE ACT OF 1934 (Amendment No. 1)* Enzo Biochem, Inc. (Name of Issuer) Common Stock, Par Value $0.01 Per Share (Title of Class of Securities) 294100102 (CUSIP Number) Sandra van der Vaart Executive Vice President, Chief Legal Officer, Chief Compliance Officer and Corporate Secretary Laboratory

July 19, 2023 424B3

ENZO BIOCHEM, INC. 1,000,000 Shares of Common Stock Issuable Upon Exercise of Warrants 2,527,808 Shares of Common Stock Issuable Upon Conversion of Debentures

Filed Pursuant to Rule 424(b)(3) Registration No. 333-272727 PROSPECTUS ENZO BIOCHEM, INC. 1,000,000 Shares of Common Stock Issuable Upon Exercise of Warrants 2,527,808 Shares of Common Stock Issuable Upon Conversion of Debentures This prospectus relates to the resale, from time to time, of up to 3,527,808 shares (the “Shares”) of our common stock, $0.01 par value per share (“Common Stock”), by th

July 18, 2023 CORRESP

July 18, 2023

July 18, 2023 VIA EDGAR AND EMAIL U.S. Securities and Exchange Commission Division of Corporation Finance Office of Industrial Applications and Services 100 F Street, N.E. Washington, D.C. 20549 Attention: Jessica Ansart Katherine Bagley Re: ENZO BIOCHEM INC Amendment No. 1 to Registration Statement on Form S-3 Filed July 10, 2023 File No. 333-272727 Dear Ms. Ansart and Ms. Bagley: I am writing to

July 18, 2023 CORRESP

Enzo Biochem, Inc. 81 Executive Blvd., Suite 3 Farmingdale, NY, 11735

Enzo Biochem, Inc. 81 Executive Blvd., Suite 3 Farmingdale, NY, 11735 July 18, 2023 VIA EDGAR Securities and Exchange Commission Division of Corporation Finance 100 F Street, N.E. Washington, DC 20549 RE: Enzo Biochem, Inc. (CIK 000316253) Registration Statement No. 333-272727 on Form S-3 (the “Registration Statement”) Ladies and Gentlemen: Enzo Biochem, Inc. (the “Registrant”) hereby requests acc

July 18, 2023 S-3/A

As filed with the Securities and Exchange Commission on July 18, 2023

As filed with the Securities and Exchange Commission on July 18, 2023 Registration No.

July 17, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): July 17, 2023 Enzo Biochem, Inc. (E

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): July 17, 2023 Enzo Biochem, Inc.

July 10, 2023 S-3/A

As filed with the Securities and Exchange Commission on July 10, 2023

As filed with the Securities and Exchange Commission on July 10, 2023 Registration No.

July 10, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): July 3, 2023 Enzo Biochem, Inc. (Ex

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): July 3, 2023 Enzo Biochem, Inc.

July 10, 2023 CORRESP

July 10, 2023

July 10, 2023 VIA EDGAR AND EMAIL U.S. Securities and Exchange Commission Division of Corporation Finance Office of Industrial Applications and Services 100 F Street, N.E. Washington, D.C. 20549 Attention: Jessica Ansart Katherine Bagley Re: ENZO BIOCHEM INC Registration Statement on Form S-3 Filed June 16, 2023 File No. 333-272727 Dear Ms. Ansart and Ms. Bagley: I am writing to submit the respons

July 10, 2023 EX-2.1

Amendment No. 1 to Asset Purchase Agreement, dated as of July 3, 2023, by and among Enzo Biochem, Inc., Enzo Clinical Labs, Inc., and Laboratory Corporation of America Holdings.

Exhibit 2.1 CONFIDENTIAL Execution Version AMENDMENT NO. 1 TO ASSET PURCHASE AGREEMENT This Amendment No. 1 to the Asset Purchase Agreement (this “Amendment”) is entered into as of July 3, 2023 (the “Amendment Effective Date”), by and among Laboratory Corporation of America Holdings, a Delaware corporation (“Buyer”), Enzo Clinical Labs, Inc., a New York corporation (“Seller”), and Enzo Biochem, In

June 29, 2023 11-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ☒ ANNUAL REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ☒ ANNUAL REPORT PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 FOR THE FISCAL YEAR ENDED DECEMBER 31, 2022 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 1

June 22, 2023 424B3

ENZO BIOCHEM, INC. Up to $30,000,000 Common Stock

Filed Pursuant to Rule 424(b)(3) Registration No. 333-271911 PROSPECTUS ENZO BIOCHEM, INC. Up to $30,000,000 Common Stock We have entered into a sales agreement with B. Riley Securities, Inc. relating to shares of our common stock offered by this prospectus. In accordance with the terms of the sales agreement, we may offer and sell shares of our common stock having an aggregate offering price of u

June 20, 2023 CORRESP

Enzo Biochem, Inc. 81 Executive Blvd., Suite 3 Farmingdale, NY, 11735

Enzo Biochem, Inc. 81 Executive Blvd., Suite 3 Farmingdale, NY, 11735 June 20, 2023 VIA EDGAR Securities and Exchange Commission Division of Corporation Finance 100 F Street, N.E. Washington, DC 20549 RE: Enzo Biochem, Inc. (CIK 000316253) Registration Statement No. 333-271911 on Form S-3 (the “Registration Statement”) Ladies and Gentlemen: Enzo Biochem, Inc. (the “Registrant”) hereby requests acc

June 16, 2023 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-3 (Form Type) Enzo Biochem, Inc.

June 16, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): June 16, 2023 Enzo Biochem, Inc. (E

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): June 16, 2023 Enzo Biochem, Inc.

June 16, 2023 S-3

As filed with the Securities and Exchange Commission on June 16, 2023

As filed with the Securities and Exchange Commission on June 16, 2023 Registration No.

June 16, 2023 S-3/A

As filed with the Securities and Exchange Commission on June 16, 2023

As filed with the Securities and Exchange Commission on June 16, 2023 Registration No.

June 16, 2023 EX-99.1

UNAUDITED PRO FORMA CONDENSED CONSOLIDATED FINANCIAL INFORMATION (in thousands, except share and per share data)

Exhibit 99.1 UNAUDITED PRO FORMA CONDENSED CONSOLIDATED FINANCIAL INFORMATION (in thousands, except share and per share data) The following unaudited pro forma condensed consolidated financial information is based upon the historical consolidated statements of Enzo Biochem, Inc., a New York corporation (the “Company”), adjusted to give effect to the sale (the “Asset Sale”) of substantially all the

June 14, 2023 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2023 ☐ TRANSITION REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2023 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM

June 9, 2023 CORRESP

Enzo Biochem, Inc. 81 Executive Blvd., Suite 3 Farmingdale, NY, 11735

Enzo Biochem, Inc. 81 Executive Blvd., Suite 3 Farmingdale, NY, 11735 June 9, 2023 VIA EDGAR Securities and Exchange Commission Division of Corporation Finance 100 F Street, N.E. Washington, DC 20549 RE: Enzo Biochem, Inc. (CIK 000316253) Registration Statement No. 333-271911 on Form S-3 (the “Registration Statement”) Ladies and Gentlemen: Enzo Biochem, Inc. (the “Registrant”) hereby requests acce

June 9, 2023 NT 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 12b-25 NOTIFICATION OF LATE FILING

NT 10-Q 1 ea180105-nt10qenzo.htm NOTIFICATION OF LATE FILING UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 12b-25 NOTIFICATION OF LATE FILING OMB APPROVAL OMB Number: 3235-0058 Estimated average burden hours per response ... 2.50 SEC FILE NUMBER CUSIP NUMBER (Check one): ☐ Form 10-K ☐ Form 20-F ☐ Form 11-K ☒ Form 10-Q ☐ Form 10-D ☐ Form N-CEN ☐ Form N-CSR For Period

May 30, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): April 6, 2023 Enzo Biochem, Inc. (E

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): April 6, 2023 Enzo Biochem, Inc.

May 26, 2023 S-3/A

As filed with the Securities and Exchange Commission on May 25, 2023

S-3/A 1 ea179145-s3a1enzobiochem.htm AMENDMENT NO. 1 TO FORM S-3 As filed with the Securities and Exchange Commission on May 25, 2023 Registration No. 333-271911 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 AMENDMENT NO. 1 TO FORM S-3 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 Enzo Biochem, Inc. (Exact name of registrant as specified in its charter) New York

May 22, 2023 EX-10.4

Form of Subsidiary Guarantee (incorporated by reference to Exhibit 10.4 of the Registrant’s Current Report on Form 8-K (File No. 001-09974) filed with the SEC on May 22, 2023)

Exhibit 10.4 Exhibit F SUBSIDIARY GUARANTEE SUBSIDIARY GUARANTEE, dated as of May 19, 2023 (this “Guarantee”), made by each of the signatories hereto (together with any other entity that may become a party hereto as provided herein, the “Guarantors”), in favor of the purchasers signatory (together with their permitted assigns, the “Purchasers”) to that certain Securities Purchase Agreement, dated

May 22, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): May 22, 2023 Enzo Biochem, Inc. (Ex

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): May 22, 2023 Enzo Biochem, Inc.

May 22, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): May 19, 2023 Enzo Biochem, Inc. (Ex

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): May 19, 2023 Enzo Biochem, Inc.

May 22, 2023 EX-10.3

Security Agreement, dated May 19, 2023, by and among Enzo Biochem, Inc., each of Enzo Biochem, Inc.’s specified subsidiaries named therein, the purchasers named therein and JGB Collateral, LLC (incorporated by reference to Exhibit 10.3 of the Registrant’s Current Report on Form 8-K (File No. 001-09974) filed with the SEC on May 22, 2023)

Exhibit 10.3 SECURITY AGREEMENT This SECURITY AGREEMENT, dated as of May 19 2023 (this “Agreement”), is among Enzo Biochem, Inc., a New York corporation (the “Company”), the Subsidiaries of the Company party hereto (such subsidiaries, the “Guarantors” and together with the Company, the “Debtors”), JGB Collateral, LLC, a Delaware limited liability company, as the collateral agent (the “Agent”), and

May 22, 2023 EX-4.2

Form of Warrant (incorporated by reference to Exhibit 4.2 of the Registrant’s Current Report on Form 8-K (File No. 001-09974) filed with the SEC on May 22, 2023)

Exhibit 4.2 NEITHER THIS SECURITY NOR THE SECURITIES FOR WHICH THIS SECURITY IS EXERCISABLE HAVE BEEN REGISTERED WITH THE SECURITIES AND EXCHANGE COMMISSION OR THE SECURITIES COMMISSION OF ANY STATE IN RELIANCE UPON AN EXEMPTION FROM REGISTRATION UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”), AND, ACCORDINGLY, MAY NOT BE OFFERED OR SOLD EXCEPT PURSUANT TO AN EFFECTIVE REGISTR

May 22, 2023 EX-10.2

Registration Rights Agreement, dated May 19, 2023, by and among Enzo Biochem, Inc. and the purchasers named therein (incorporated by reference to Exhibit 10.2 of the Registrant’s Current Report on Form 8-K (File No. 001-09974) filed with the SEC on May 22, 2023)

Exhibit 10.2 REGISTRATION RIGHTS AGREEMENT This Registration Rights Agreement (this “Agreement”) is made and entered into as of May 19, 2023, between Enzo Biochem, Inc., a New York corporation (the “Company”), and each of the several purchasers signatory hereto (each such purchaser, a “Purchaser” and, collectively, the “Purchasers”). This Agreement is made pursuant to the Securities Purchase Agree

May 22, 2023 EX-10.1

Securities Purchase Agreement, dated May 19, 2023, by and among Enzo Biochem, Inc., the purchasers named therein, and JGB Collateral, LLC (incorporated by reference to Exhibit 10.1 of the Registrant’s Current Report on Form 8-K (File No. 001-09974) filed with the SEC on May 22, 2023)

Exhibit 10.1 SECURITIES PURCHASE AGREEMENT This Securities Purchase Agreement (this “Agreement”) is dated as of May 19, 2023, between Enzo Biochem, Inc., a New York corporation (the “Company”), each purchaser identified on the signature pages hereto (each, including its successors and assigns, a “Purchaser” and collectively, the “Purchasers”), and JGB Collateral LLC, a Delaware limited liability c

May 22, 2023 EX-4.1

Form of Debenture (incorporated by reference to Exhibit 4.1 of the Registrant’s Current Report on Form 8-K (File No. 001-09974) filed with the SEC on May 22, 2023)

Exhibit 4.1 NEITHER THIS SECURITY NOR THE SECURITIES INTO WHICH THIS SECURITY IS CONVERTIBLE HAVE BEEN REGISTERED WITH THE SECURITIES AND EXCHANGE COMMISSION OR THE SECURITIES COMMISSION OF ANY STATE IN RELIANCE UPON AN EXEMPTION FROM REGISTRATION UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”), AND, ACCORDINGLY, MAY NOT BE OFFERED OR SOLD EXCEPT PURSUANT TO AN EFFECTIVE REGIST

May 15, 2023 EX-1.2

Sales Agreement, dated May 12, 2023, between Enzo Biochem, Inc. and B. Riley Securities, Inc.

Exhibit 1.2 ENZO BIOCHEM, INC. Common Stock (par value $0.01 per share) At Market Issuance Sales Agreement May 12, 2023 B. Riley Securities, Inc. 299 Park Avenue, 21st Floor New York, NY 10171 Ladies and Gentlemen: Enzo Biochem, Inc., a New York corporation (the “Company”), confirms its agreement (this “Agreement”) with B. Riley Securities, Inc. (the “Agent”) as follows: 1. Issuance and Sale of Sh

May 15, 2023 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-3 (Form Type) Enzo Biochem, Inc.

May 15, 2023 S-3

Power of Attorney (contained on page II-5)

As filed with the Securities and Exchange Commission on May 12, 2023 Registration No.

May 10, 2023 SC 13D/A

ENZ / Enzo Biochem, Inc. / Radoff Bradley Louis - AMENDMENT NO. 4 TO SCHEDULE 13D Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO § 240.13d-1(a) AND AMENDMENTS THERETO FILED PURSUANT TO § 240.13d-2(a) (Amendment No. 4)1 Enzo Biochem, Inc. (Name of Issuer) Common Stock, par value $0.01 per share (Title of Class of Securities) 294100102 (CUSIP Number) BRADLEY L. RADOFF 2

May 10, 2023 EX-99.1

SUPPORT AGREEMENT

Exhibit 99.1 SUPPORT AGREEMENT This SUPPORT AGREEMENT (this “Agreement”), dated as of [•], 2023, is by and between Laboratory Corporation of America Holdings, a Delaware corporation (“Buyer”) and [Stockholder Name] (the “Stockholder”). WHEREAS, as of the date hereof, the Stockholder is the record and beneficial owner (as defined in Rule 13d-3 under the Securities Exchange Act of 1934 (the “Exchang

May 8, 2023 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Defin

May 8, 2023 8-K

Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): May 8, 2023 Enzo Biochem, Inc.

April 24, 2023 DEFM14A

Schedule 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Defin

April 24, 2023 DEFA14A

Schedule 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Defin

April 13, 2023 PREM14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☒ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Defin

April 13, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): April 6, 2023 Enzo Biochem, Inc. (E

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): April 6, 2023 Enzo Biochem, Inc.

April 13, 2023 EX-FILING FEES

Calculation of Filing Fee Tables Schedule 14A (Form Type) Enzo Biochem, Inc. (Exact Name of Registrant as Specified in its Charter) Table 1: Transaction Valuation

Exhibit 107 Calculation of Filing Fee Tables Schedule 14A (Form Type) Enzo Biochem, Inc.

April 5, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 31, 2023 Enzo Biochem, Inc. (

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 31, 2023 Enzo Biochem, Inc.

April 5, 2023 EX-10.1

Credit Agreement, dated as of March 31, 2023, by and among Enzo Clinical Labs, Inc., Enzo Life Sciences, Inc., Gemino Healthcare Finance, LLC d/b/a SLR Healthcare ABL, Enzo Biochem, Inc. and certain other parties thereto.

Exhibit 10.1 CREDIT AGREEMENT by and among ENZO CLINICAL LABS, INC., ENZO LIFE SCIENCES, INC., such other Persons joined hereto as a Borrower from time to time, as Borrowers, and GEMINO HEALTHCARE FINANCE, LLC d/b/a SLR HEALTHCARE ABL, as Lender Dated as of March 31, 2023 TABLE OF CONTENTS ARTICLE 1 DEFINITIONS, ACCOUNTING TERMS AND PRINCIPLES OF CONSTRUCTION 1 1.01 Terms Defined 1 1.02 Accounting

March 27, 2023 SC 13D

ENZ / Enzo Biochem, Inc. / Laboratory Corp Of America Holdings - SC 13D Activist Investment

SC 13D SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D UNDER THE SECURITIES EXCHANGE ACT OF 1934 (Amendment No. )* Enzo Biochem, Inc. (Name of Issuer) Common Stock, Par Value $0.01 Per Share (Title of Class of Securities) 294100102 (CUSIP Number) Sandra van der Vaart Executive Vice President, Chief Legal Officer, Chief Compliance Officer and Corporate Secretary Laboratory Co

March 20, 2023 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2023 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2023 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

March 16, 2023 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 16, 2023 Enzo Biochem, Inc. (

DEFA14A 1 ea175132-8kenzobiochem.htm CURRENT REPORT UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 16, 2023 Enzo Biochem, Inc. (Exact Name of Registrant as Specified in Its Charter) New York (State or Other Jurisdiction of Inc

March 16, 2023 EX-2.1

Asset Purchase Agreement, dated as of March 16, 2023, by and among Laboratory Corporation of America Holdings, Enzo Clinical Labs, Inc. and Enzo Biochem, Inc.

Exhibit 2.1 ASSET PURCHASE AGREEMENT dated as of March 16, 2023 by and among LABORATORY CORPORATION OF AMERICA HOLDINGS, Enzo Clinical Labs, Inc. and ENZO BIOCHEM, INC. TABLE OF CONTENTS Page Article I DEFINITIONS 2 1.1 Definitions 2 1.2 Other Definitional Provisions 23 Article II SALE AND PURCHASE OF ACQUIRED ASSETS 24 2.1 Transfer of Acquired Assets 24 2.2 The Closing 24 2.3 Purchase Price 24 2.

March 16, 2023 EX-99.1

Press Release, dated March 16, 2023, issued by Enzo Biochem, Inc.

Exhibit 99.1 Enzo Biochem Agrees to Sell Clinical Laboratory to Labcorp Significant value unlocked for Enzo shareholders with continued focus on remaining businesses. FARMINGDALE, NY, March 16, 2023 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE:ENZ) announced today that it has entered into an agreement for Labcorp (NYSE: LH) to acquire the assets of Enzo’s Clinical Laboratory division (Enzo Clinical

March 16, 2023 EX-99.1

Enzo Biochem Agrees to Sell Clinical Laboratory to Labcorp Significant value unlocked for Enzo shareholders with continued focus on remaining businesses.

Exhibit 99.1 Enzo Biochem Agrees to Sell Clinical Laboratory to Labcorp Significant value unlocked for Enzo shareholders with continued focus on remaining businesses. FARMINGDALE, NY, March 16, 2023 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE:ENZ) announced today that it has entered into an agreement for Labcorp (NYSE: LH) to acquire the assets of Enzo’s Clinical Laboratory division (Enzo Clinical

March 16, 2023 EX-2.1

Asset Purchase Agreement, dated as of March 16, 2023, by and among Laboratory Corporation of America Holdings, Enzo Clinical Labs, Inc. and Enzo Biochem, Inc. (incorporated by reference to Exhibit 2.1 to the Issuer’s Current Report on Form 8-K, filed with the SEC on March 16, 2023).

Exhibit 2.1 ASSET PURCHASE AGREEMENT dated as of March 16, 2023 by and among LABORATORY CORPORATION OF AMERICA HOLDINGS, Enzo Clinical Labs, Inc. and ENZO BIOCHEM, INC. TABLE OF CONTENTS Page Article I DEFINITIONS 2 1.1 Definitions 2 1.2 Other Definitional Provisions 23 Article II SALE AND PURCHASE OF ACQUIRED ASSETS 24 2.1 Transfer of Acquired Assets 24 2.2 The Closing 24 2.3 Purchase Price 24 2.

March 16, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 16, 2023 Enzo Biochem, Inc. (

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 16, 2023 Enzo Biochem, Inc.

March 14, 2023 NT 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 12b-25 NOTIFICATION OF LATE FILING

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 12b-25 NOTIFICATION OF LATE FILING OMB APPROVAL OMB Number: 3235-0058 Estimated average burden hours per response ... 2.50 SEC FILE NUMBER CUSIP NUMBER (Check one): ☐ Form 10-K ☐ Form 20-F ☐ Form 11-K ☒ Form 10-Q ☐ Form 10-D ☐ Form N-CEN ☐ Form N-CSR For Period Ended: January 31, 2023 ☐ Transition Report on Form 10-K ☐ Tr

February 3, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): January 31, 2023 Enzo Biochem, Inc.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): January 31, 2023 Enzo Biochem, Inc.

January 23, 2023 SC 13D/A

ENZ / Enzo Biochem, Inc. / Wolf James G. - SC 13D/A Activist Investment

SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 13D/A (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO §240.

December 21, 2022 DEFR14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. 1)

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. 1) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Defi

December 21, 2022 DEF 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Defin

December 12, 2022 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2022 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2022 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

November 25, 2022 10-K/A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 1) (Mark One) ☒ Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2022 ☐ Transiti

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 1) (Mark One) ? Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2022 or ? Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the transition period from to Commission File Number: 001-0997

November 23, 2022 SC 13D/A

ENZ / Enzo Biochem, Inc. / Wolf James G. - SC 13D/A Activist Investment

SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 13D/A (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO ?240.

November 4, 2022 EX-10.2

Amended and Restated Employment Agreement with Hamid Erfanian (b)

Exhibit 10.2 SECOND AMENDED AND RESTATED EXECUTIVE EMPLOYMENT AGREEMENT This Second Amended and Restated Executive Employment Agreement (the “Agreement”), dated as of October 14, 2021 (the “Effective Date”), as amended March 24, 2022 and further amended October 20, 2022 (the “Amendment Date”), made between Enzo Biochem, Inc., a New York corporation, with its principal office at 527 Madison Avenue,

November 4, 2022 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): November 3, 2022 (October 20, 2022)

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): November 3, 2022 (October 20, 2022) Enzo Biochem, Inc.

November 4, 2022 EX-10.1

Amended and Restated Employment Agreement with Kara Cannon (a)

Exhibit 10.1 FIRST AMENDED AND RESTATED EXECUTIVE EMPLOYMENT AGREEMENT This Executive Employment Agreement (the “Agreement”), dated as of March 21, 2022 (the “Effective Date”), as amended October 20, 2022 (the Amendment Date”), made between Enzo Biochem, Inc., a New York corporation, with its principal office at 527 Madison Avenue, New York, New York 10022 (the “Company”) and Kara Cannon (the “Exe

October 26, 2022 SC 13D/A

ENZ / Enzo Biochem, Inc. / Wolf James G. - SC 13D/A Activist Investment

SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 13D/A (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO §240.

October 20, 2022 EX-99.1

Enzo Biochem, Inc. Appoints Patricia Eckert, CPA as Interim CFO - David Bench resigning to pursue a new career opportunity -

Exhibit 99.1 Enzo Biochem, Inc. Appoints Patricia Eckert, CPA as Interim CFO - David Bench resigning to pursue a new career opportunity - NEW YORK, NY, October 20, 2022 ? Enzo Biochem, Inc. (NYSE: ENZ) (?Enzo? or the ?Company?), a leading biosciences and diagnostics company, today announced the appointment of Patricia Eckert, CPA, as interim Chief Financial Officer (CFO). Ms. Eckert?s appointment

October 20, 2022 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): October 20, 2022 (October 15, 202

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event reported): October 20, 2022 (October 15, 2022) ENZO BIOCHEM, INC. (Exact Name of Registrant as Specified in its Charter) New York 001-09974 13-2866202 (State or Other Jurisdiction of Incorporati

October 14, 2022 EX-32.A

Certification of CEO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

EXHIBIT 32 (a) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2022 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Hamid Erfanian, Chief Executive

October 14, 2022 EX-31.B

Certification of CFO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

EXHIBIT 31 (b) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

October 14, 2022 EX-10.AE

Sublease agreement between Enzo Biochem, Inc. and Siemens Corporation

Exhibit 10(ae)

October 14, 2022 EX-21

List of subsidiaries of the Company

EXHIBIT 21 List of subsidiaries of the Company Enzo Clinical Labs, Inc., a New York Corporation Enzo Life Sciences, Inc., a New York Corporation Enzo Life Sciences (ELS) AG, in Lausen, Switzerland, a wholly-owned subsidiary of Enzo Life Sciences, Inc. Enzo Therapeutics, Inc., a New York Corporation Enzo Realty LLC, a New York Corporation Enzo Realty II, LLC, a New York Corporation

October 14, 2022 EX-32.B

Certification of CFO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

EXHIBIT 32 (b) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2022 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, David Bench, Chief Financial Of

October 14, 2022 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2022 ☐ TRANSITION REPORT PURSUANT TO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2022 or ☐ TRANSITION REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM, INC.

October 14, 2022 EX-31.A

Certification of CEO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

EXHIBIT 31 (a) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

June 24, 2022 11-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ☒ ANNUAL REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ? ANNUAL REPORT PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 FOR THE FISCAL YEAR ENDED DECEMBER 31, 2021 OR ? TRANSITION REPORT PURSUANT TO SECTION 1

June 9, 2022 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2022 ☐ TRANSITION REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ? QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2022 or ? TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM

May 6, 2022 8-K/A

Amendments to Articles of Incorporation or Bylaws; Change in Fiscal Year, Material Modification to Rights of Security Holders, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K/A Amendment No.

May 6, 2022 EX-3.1

Amended and Restated Certificate of Incorporation of Enzo Biochem, Inc.

Exhibit 3.1 RESTATED CERTIFICATE OF INCORPORATION OF ENZO BIOCHEM, INC. Under Section 807 of the Business Corporation Law The undersigned, for the purpose of restating the corporation?s Certificate of Incorporation, pursuant to Section 807 of the Business Corporation Law of the State of New York, does hereby certify: FIRST: The name under which the corporation was formed is Enzo Biochem, Inc. SECO

May 6, 2022 EX-3.2

Amended and Restated By-Laws of Enzo Biochem, Inc.

Exhibit 3.2 AMENDED AND RESTATED BY-LAWS OF ENZO BIOCHEM, INC. (a New York Corporation) (as amended and restated as of April 25, 2022) ARTICLE I MEETING OF SHAREHOLDERS Section 1. Annual Meeting. The annual meeting of the shareholders of Enzo Biochem, Inc., (hereinafter called the ?Corporation?) for the election of directors and for the transaction of such other business as may come before the mee

April 27, 2022 8-K

Amendments to Articles of Incorporation or Bylaws; Change in Fiscal Year, Material Modification to Rights of Security Holders, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): April 25, 2022 Enzo Biochem, Inc.

April 27, 2022 EX-3.2

Amended and restated Bylaws (34)

Exhibit 3.2 AMENDED AND RESTATED BY-LAWS OF ENZO BIOCHEM, INC. (a New York Corporation) (as amended and restated as of April 25, 2022) ARTICLE I MEETING OF SHAREHOLDERS Section 1. Annual Meeting. The annual meeting of the shareholders of Enzo Biochem, Inc., (hereinafter called the ?Corporation?) for the election of directors and for the transaction of such other business as may come before the mee

April 27, 2022 EX-3.1

Restated Certificate of Incorporation (1)

Exhibit 3.1 RESTATED CERTIFICATE OF INCORPORATION OF ENZO BIOCHEM, INC. Under Section 807 of the Business Corporation Law The undersigned, for the purpose of restating the corporation?s Certificate of Incorporation, pursuant to Section 807 of the Business Corporation Law of the State of New York, does hereby certify: FIRST: The name under which the corporation was formed is Enzo Biochem, Inc. SECO

April 8, 2022 8-K

Submission of Matters to a Vote of Security Holders

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): April 8, 2022 Enzo Biochem, Inc.

April 6, 2022 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

DEFA14A 1 ea158137-defa14aenzobio.htm DEFINITIVE ADDITIONAL MATERIALS UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for

April 1, 2022 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ? Filed by a Party other than the Registrant ? Check the appropriate box: ? Preliminary Proxy Statement ? Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ? Definitive Proxy Statement ? Definitive Additional Materials ? Soliciting Material Under Rule 14a-12 Enzo Biochem, Inc.

April 1, 2022 8-K

Submission of Matters to a Vote of Security Holders

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 31, 2022 Enzo Biochem, Inc.

March 30, 2022 EX-10.3

First Amended and Restated Employment Agreement between Enzo Biochem, Inc. and Hamid Erfanian, effective as of March 24, 2022

Exhibit 10.3 FIRST AMENDED AND RESTATED EXECUTIVE EMPLOYMENT AGREEMENT This First Amended and Restated Executive Employment Agreement (the ?Agreement?), dated as of October 14, 2021, as amended March 24, 2022, made between Enzo Biochem, Inc., a New York corporation, with its principal office at 527 Madison Avenue, New York, New York 10022, (the ?Company?) and Hamid Erfanian (the ?Executive?) (coll

March 30, 2022 8-K

Departure of Directors or Certain Officers; Election of Directors; Appointment of Certain Officers; Compensatory Arrangements of Certain Officers, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 24, 2022 Enzo Biochem, Inc.

March 30, 2022 EX-99.1

Enzo Biochem Announces Appointments of David Bench and Kara Cannon to New Executive Management Positions

Exhibit 99.1 Enzo Biochem, Inc. 527 Madison Avenue New York, NY 10022 FOR IMMEDIATE RELEASE Enzo Biochem Announces Appointments of David Bench and Kara Cannon to New Executive Management Positions NEW YORK, NY, March 28, 2022 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (?Enzo? or the ?Company?), a leading biosciences and diagnostics company, today announced that Kara Cannon, formerly Chief C

March 30, 2022 EX-10.1

Employment Agreement between Enzo Biochem, Inc. and David Bench, effective as of March 24, 2022

Exhibit 10.1 EXECUTIVE EMPLOYMENT AGREEMENT This Executive Employment Agreement (the ?Agreement?), dated as of March 24, 2022, made between Enzo Biochem, Inc., a New York corporation, with its principal office at 527 Madison Avenue, New York, New York 10022, (the ?Company?) and David Bench (the ?Executive?) (collectively, the ?Parties?). Whereas, Executive is currently a full-time employee of the

March 30, 2022 EX-10.2

Employment Agreement between Enzo Biochem, Inc. and Kara Cannon, effective as of March 24, 2022

Exhibit 10.2 EXECUTIVE EMPLOYMENT AGREEMENT This Executive Employment Agreement (the ?Agreement?), dated as of March 24, 2022, made between Enzo Biochem, Inc., a New York corporation, with its principal office at 527 Madison Avenue, New York, New York 10022 (the ?Company?) and Kara Cannon (the ?Executive?) (collectively, the ?Parties?). Whereas, Executive is currently a full-time employee of the C

March 25, 2022 SC 13D/A

ENZ / Enzo Biochem, Inc. / Roumell Asset Management, LLC - AMENDMENT NO. 16 TO SCHEDULE 13D Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. 16) Enzo Biochem, Inc. (Name of Issuer) Common Stock (Title of Class of Securities) 294100102 (CUSIP Number) James C. Roumell Asset Management, LLC 2 Wisconsin Circle, Suite 700 Chevy Chase, MD 20815 (301) 656-8500 (Name, Address and Telephone Number of Pers

March 25, 2022 EX-99.1

* * * * *

Exhibit 99.1 March 24, 2022 Via E-mail Craig L. Lukin c/o: Roumell Asset Management, LLC 2 Wisconsin Cir #700 Chevy Chase, Maryland 20815 Email: [email protected] Re: Termination of Joint Filing and Solicitation Agreement, dated September 29, 2021 Dear Craig: The undersigned constitute all the parties to that certain Joint Filing and Solicitation Agreement, dated September 29, 2021 (the ?Agr

March 18, 2022 8-K

Amendments to Articles of Incorporation or Bylaws; Change in Fiscal Year, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 11, 2022 Enzo Biochem, Inc.

March 18, 2022 EX-3.1

Amendment No. 2 to Amended and Restated By-Laws of Enzo Biochem, Inc., effective as of March 11, 2022

Exhibit 3.1 AMENDMENT NO. 2 TO AMENDED AND RESTATED BY-LAWS OF ENZO BIOCHEM, INC. (a New York corporation) Article I, Section 8 of the Amended and Restated By-Laws of Enzo Biochem, Inc. is hereby amended and replaced with the following: Section 8. Order of Business. The order of business at all meetings of the shareholders shall be as determined by the chairman of the meeting. The Board may adjour

March 14, 2022 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2022 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ? QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended January 31, 2022 or ? TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

March 7, 2022 8-K

Departure of Directors or Certain Officers; Election of Directors; Appointment of Certain Officers; Compensatory Arrangements of Certain Officers

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): Februrary 25, 2022 Enzo Biochem, Inc.

March 1, 2022 DEF 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ? Filed by a party other than the Registrant ? Check the appropriate box: ? Preliminary Proxy Statement ? Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ? Defin

February 18, 2022 PRER14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 14A (Amendment No. 1) Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 14A (Amendment No.

February 10, 2022 SC 13G/A

ENZ / Enzo Biochem, Inc. / VANGUARD GROUP INC - SCHEDULE 13G/A Passive Investment

SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 13G Under the Securities Exchange Act of 1934 (Amendment No.: 1)* Name of issuer: Enzo Biochem Inc. Title of Class of Securities: Common Stock CUSIP Number: 294100102 Date of Event Which Requires Filing of this Statement: December 31, 2021 Check the appropriate box to designate the rule pursuant to which this Schedule is filed: ??R

January 21, 2022 8-K

Departure of Directors or Certain Officers; Election of Directors; Appointment of Certain Officers; Compensatory Arrangements of Certain Officers

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): January 21, 2022 Enzo Biochem, Inc.

January 18, 2022 SC 13G/A

ENZ / Enzo Biochem, Inc. / Evermore Global Advisors, LLC - SC 13G/A Passive Investment

SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G Under the Securities Exchange Act of 1934 (Amendment No. 7)* Enzo Biochem, Inc. (Name of Issuer) Common Stock, $0.01 Par Value (Title of Class of Securities) 294100102 (CUSIP Number) December 31, 2021 (Date of Event Which Requires Filing of this Statement) Check the appropriate box to designate the rule pursuant to which this S

January 6, 2022 PRE 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ? Filed by a Party other than the Registrant ? Check the appropriate box: ? Preliminary Proxy Statement ? Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ? Definitive Proxy Statement ? Definitive Additional Materials ? Soliciting Material Under Rule 14a-12 Enzo Biochem, Inc.

January 4, 2022 EX-99.1

COOPERATION AGREEMENT

Exhibit 99.1 Execution Version COOPERATION AGREEMENT This Cooperation Agreement (this ?Agreement?) is made and entered into as of January 3, 2022 by and among Enzo Biochem, Inc. (the ?Company?) and the entities and natural persons set forth in the signature pages hereto (collectively, the ?Radoff Parties?) (each of the Company and the Radoff Parties, a ?Party? to this Agreement, and collectively,

January 4, 2022 8-K

Regulation FD Disclosure, Departure of Directors or Certain Officers; Election of Directors; Appointment of Certain Officers; Compensatory Arrangements of Certain Officers, Entry into a Material Definitive Agreement, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): January 3, 2022 Enzo Biochem, Inc.

January 4, 2022 EX-10.1

Cooperation agreement by and among Enzo Biochem, Inc. and the Radoff Parties (10)

Exhibit 10.1 COOPERATION AGREEMENT This Cooperation Agreement (this ?Agreement?) is made and entered into as of January 3, 2022 by and among Enzo Biochem, Inc. (the ?Company?) and the entities and natural persons set forth in the signature pages hereto (collectively, the ?Radoff Parties?) (each of the Company and the Radoff Parties, a ?Party? to this Agreement, and collectively, the ?Parties?). RE

January 4, 2022 EX-99.2

JOINT FILING AGREEMENT

Exhibit 99.2 JOINT FILING AGREEMENT In accordance with Rule 13d-1(k)(1)(iii) under the Securities Exchange Act of 1934, as amended, the persons named below agree to the joint filing on behalf of each of them of a Statement on Schedule 13D (including additional amendments thereto) with respect to the common stock, par value $0.01 per share, of Enzo Biochem, Inc., a New York corporation. This Joint

January 4, 2022 SC 13D/A

ENZ / Enzo Biochem, Inc. / Radoff Bradley Louis Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO ? 240.13d-1(a) AND AMENDMENTS THERETO FILED PURSUANT TO ? 240.13d-2(a) (Amendment No. 3)1 Enzo Biochem, Inc. (Name of Issuer) Common Stock, par value $0.01 per share (Title of Class of Securities) 294100102 (CUSIP Number) BRADLEY L. RADOFF 2

January 4, 2022 EX-99.1

Hamid Erfanian and Bradley Radoff Appointed to Enzo Biochem’s Board of Directors

Exhibit 99.1 Enzo Biochem, Inc. 527 Madison Avenue New York, NY 10022 FOR IMMEDIATE RELEASE Hamid Erfanian and Bradley Radoff Appointed to Enzo Biochem?s Board of Directors NEW YORK, NY, January 3, 2022 (GLOBE NEWSWIRE) - Enzo Biochem, Inc. (NYSE: ENZ) (?Enzo? or the ?Company?), a leading biosciences and diagnostics company, today announced the appointments of Hamid Erfanian and Bradley Radoff to

December 27, 2021 SC 13D/A

ENZ / Enzo Biochem, Inc. / Radoff Bradley Louis Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO § 240.13d-1(a) AND AMENDMENTS THERETO FILED PURSUANT TO § 240.13d-2(a) (Amendment No. 2)1 Enzo Biochem, Inc. (Name of Issuer) Common Stock, par value $0.01 per share (Title of Class of Securities) 294100102 (CUSIP Number) BRADLEY L. RADOFF 2

December 15, 2021 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2021 ☐ TRANSITION REPORT P

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ? QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended October 31, 2021 or ? TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCH

December 15, 2021 EX-10.1

Executive Employment Agreement between the Company and Hamid Erfanian, dated October 14, 2021.

Exhibit 10.1 EXECUTIVE EMPLOYMENT AGREEMENT This Executive Employment Agreement (the ?Agreement?), dated as of October 14, 2021, made between Enzo Biochem, Inc., a New York corporation, with its principal office at 527 Madison Avenue, New York, New York 10022, (the ?Company?) and Hamid Erfanian (the ?Executive?) (collectively, the ?Parties?). Whereas, the Company desires for Executive to provide s

December 2, 2021 CORRESP

December 2, 2021

O L S H A N 1325 AVENUE OF THE AMERICAS ● NEW YORK, NEW YORK 10019 TELEPHONE: 212.

December 2, 2021 PRRN14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Ame

PRRN14A 1 prrn14a10907604712022021.htm UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Amendment No. 1) Filed by the Registrant ☐ Filed by a Party other than the Registrant ☒ Check the appropriate box:

December 1, 2021 10-K/A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 2) (Mark One) ☒ Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2021 ☐ Transiti

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 2) (Mark One) ? Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2021 or ? Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the transition period from to Commission File Number: 001-0997

November 29, 2021 10-K/A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 1) (Mark One) ☒ Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2021 ☐ Transiti

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K/A (Amendment No. 1) (Mark One) ? Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the fiscal year ended July 31, 2021 or ? Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 For the transition period from to Commission File Number: 001-0997

November 19, 2021 PREC14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Ame

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ? Filed by a Party other than the Registrant ? Check the appropriate box: ? Preliminary Proxy Statement ? Confide

November 15, 2021 SC 13D/A

ENZ / Enzo Biochem, Inc. / Wolf James G. - SCHEDULE 13D/A Activist Investment

SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 13D/A (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO ?240.

November 9, 2021 EX-99.1

Inducement Restricted Stock Unit Grant Notice and Agreement

Exhibit 99.1 INDUCEMENT RESTRICTED STOCK UNIT GRANT NOTICE AND AGREEMENT To: Hamid Erfanian (referred to herein as ?Grantee? or ?you?) WHEREAS, Enzo Biochem, Inc. (the ?Company?) desires to employ the Grantee as its Chief Executive Officer; WHEREAS, in an Executive Employment Agreement dated October 14, 2021 (the ?Employment Agreement?), the Company has agreed to grant the Grantee an inducement re

November 9, 2021 S-8

As filed with the Securities and Exchange Commission on November 9, 2021

As filed with the Securities and Exchange Commission on November 9, 2021 Registration No.

November 9, 2021 EX-99.2

Inducement Option Grant Notice and Agreement

Exhibit 99.2 INDUCEMENT STOCK OPTION GRANT NOTICE AND AGREEMENT To: Hamid Erfanian (referred to herein as ?Grantee? or ?you?) WHEREAS, Enzo Biochem, Inc. (the ?Company?) desires to employ the Grantee as its Chief Executive Officer; WHEREAS, in an Executive Employment Agreement dated October 14, 2021 (the ?Employment Agreement?), the Company has agreed to grant the Grantee an inducement stock optio

October 28, 2021 SC 13D/A

ENZ / Enzo Biochem, Inc. / Harbert Discovery Fund, LP - ENZO BIOCHEM, INC. Activist Investment

SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D/A Under the Securities Exchange Act of 1934 (Amendment No. 14)* Enzo Biochem, Inc. (Name of Issuer) Common Stock, $0.01 par value (Title of Class of Securities) 294100102 (CUSIP Number) Kevin A. McGovern, Esq. c/o Harbert Discovery Fund, LP 2100 Third Avenue North, Suite 600 Birmingham, AL 35203 (205) 987-5577 with a copy to: E

October 25, 2021 DFAN14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Ame

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ? Filed by a Party other than the Registrant ? Check the appropriate box: ? Preliminary Proxy Statement ? Confide

October 22, 2021 SC 13D/A

ENZ / Enzo Biochem, Inc. / Radoff Bradley Louis Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO § 240.13d-1(a) AND AMENDMENTS THERETO FILED PURSUANT TO § 240.13d-2(a) (Amendment No. 1)1 Enzo Biochem, Inc. (Name of Issuer) Common Stock, par value $0.01 per share (Title of Class of Securities) 294100102 (CUSIP Number) BRADLEY L. RADOFF 2

October 19, 2021 DFAN14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Ame

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (Rule 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of The Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ? Filed by a Party other than the Registrant ? Check the appropriate box: ? Preliminary Proxy Statement ? Confide

October 18, 2021 8-K

Departure of Directors or Certain Officers; Election of Directors; Appointment of Certain Officers; Compensatory Arrangements of Certain Officers, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): October 14, 2021 Enzo Biochem, Inc.

October 18, 2021 EX-99.1

HAMID ERFANAIN NAMED CHIEF EXECUTIVE OFFICER OF ENZO BIOCHEM TO ACCELERATE EXPANSION AND COMMERCIALIZATION OF KEY DIAGNOSTIC PLATFORMS Dr. Elazar Rabbani to remain Chairperson of the Board and will assume new role as Chief Scientific Officer

Exhibit 99.1 Enzo Biochem, Inc. 527 Madison Avenue New York, NY 10022 FOR IMMEDIATE RELEASE HAMID ERFANAIN NAMED CHIEF EXECUTIVE OFFICER OF ENZO BIOCHEM TO ACCELERATE EXPANSION AND COMMERCIALIZATION OF KEY DIAGNOSTIC PLATFORMS Dr. Elazar Rabbani to remain Chairperson of the Board and will assume new role as Chief Scientific Officer NEW YORK, NY, October 18, 2021 (GLOBE NEWSWIRE) - Enzo Biochem, In

October 12, 2021 EX-31.B

Certification of CFO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

EXHIBIT 31 (b) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

October 12, 2021 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2021 ☐ TRANSITION REPORT PURSUANT TO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 10-K (Mark one) ? ANNUAL REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended July 31, 2021 or ? TRANSITION REPORT PURSUANT TO SECTION 13 or 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM, INC.

October 12, 2021 EX-21

List of subsidiaries of the Company

Exhibit 21 List of subsidiaries of the Company Enzo Clinical Labs, Inc., a New York Corporation Enzo Life Sciences, Inc., a New York Corporation Enzo Life Sciences (ELS) AG, in Lausen, Switzerland, a wholly-owned subsidiary of Enzo Life Sciences, Inc. Enzo Therapeutics, Inc., a New York Corporation Enzo Realty LLC, a New York Corporation Enzo Realty II, LLC, a New York Corporation

October 12, 2021 EX-32.B

Certification of CFO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

EXHIBIT 32 (b) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2021 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, David Bench, Chief Financial Of

October 12, 2021 EX-31.A

Certification of CEO Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

EXHIBIT 31 (a) CERTIFICATIONS In connection with the Annual Report on Form 10-K of Enzo Biochem, Inc.

October 12, 2021 EX-32.A

Certification of CEO Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

EXHIBIT 32 (a) CERTIFICATE PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 In connection with the Annual Report of Enzo Biochem, Inc., and Subsidiaries (“the Company”) on Form 10-K for the fiscal year ended July 31, 2021 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Elazar Rabbani, Ph.D., Chief Ex

October 6, 2021 EX-99.1

Group Agreement, dated September 29, 2021.

GROUP AGREEMENT WHEREAS, certain of the undersigned are shareholders, direct or beneficial, of Enzo Biochem, Inc.

October 6, 2021 SC 13D

ENZ / Enzo Biochem, Inc. / Radoff Bradley Louis - THE SCHEDULE 13D Activist Investment

SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 13D (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO RULE 13d-1(a) AND AMENDMENTS THERETO FILED PURSUANT TO RULE 13d-2(a) (Amendment No.

October 6, 2021 EX-99.2

Power of Attorney

POWER OF ATTORNEY Know all by these presents, that the undersigned hereby constitutes and appoints Bradley L.

October 1, 2021 SC 13D

ENZ / Enzo Biochem, Inc. / Wolf James G. - SC 13D Activist Investment

SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 SCHEDULE 13D (Rule 13d-101) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO ?240.

September 30, 2021 EX-99.2

JOINT FILING AND SOLICITATION AGREEMENT

Exhibit 99.2 JOINT FILING AND SOLICITATION AGREEMENT WHEREAS, certain of the undersigned are stockholders, direct or beneficial, of Enzo BioChem, Inc., a New York corporation (the ?Company?); WHEREAS, Roumell Opportunistic Value Fund (the ?Fund?), Roumell Asset Management, LLC, a Maryland limited liability company (?Roumell?), James C. Roumell, Susanne L. Meline and Edward Terino wish to form a gr

September 30, 2021 EX-99.3

ROUMELL ASSET MANAGEMENT, LLC 2 Wisconsin Circle, Suite 700 Chevy Chase, MD 20815

Exhibit 99.3 ROUMELL ASSET MANAGEMENT, LLC 2 Wisconsin Circle, Suite 700 Chevy Chase, MD 20815 September [?], 2021 [] Re: Enzo Biochem, Inc. Dear []: Thank you for agreeing to serve as a nominee for election to the Board of Directors of Enzo Biochem, Inc. (the ?Company?) in connection with the proxy solicitation that Roumell Opportunistic Value Fund (the ?Fund?) is considering undertaking to nomin

September 30, 2021 EX-99.1

Termination of Joint Filing and Solicitation Agreement, dated September 29, 2021

Exhibit 99.1 September 29, 2021 Via E-mail Craig L. Lukin c/o: Roumell Asset Management, LLC 2 Wisconsin Cir #700 Chevy Chase, Maryland 20815 Email: [email protected] Re: Termination of Joint Filing and Solicitation Agreement, dated November 27, 2020 Dear Craig: The undersigned constitute all the parties to that certain Joint Filing and Solicitation Agreement, dated November 27, 2020 (the ?A

September 30, 2021 SC 13D/A

ENZ / Enzo Biochem, Inc. / Roumell Asset Management, LLC - AMENDMENT NO. 15 TO SCHEDULE 13D Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. 15) Enzo Biochem, Inc. (Name of Issuer) Common Stock (Title of Class of Securities) 294100102 (CUSIP Number) James C. Roumell Asset Management, LLC 2 Wisconsin Circle, Suite 700 Chevy Chase, MD 20815 (301) 656-8500 (Name, Address and Telephone Number of Pers

August 20, 2021 SC 13G

ENZ / Enzo Biochem, Inc. / Wolf James G. - SC 13G Passive Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G (Rule 13d-102) INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT TO §240.13d-1(b), (c) AND (d) AND AMENDMENTS THERETO FILED PURSUANT §240.13d-2 (Amendment No. ) ENZO BIOCHEM, INC. (Name of Issuer) Common Stock $.01 Par Value (Title of Class of Securities) 294100102 (CUSIP Number) August 10, 2021 (Date of Eve

June 25, 2021 11-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM 11-K FOR ANNUAL REPORTS OF EMPLOYEE STOCK PURCHASE, SAVINGS AND SIMILAR PLANS PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 (MARK ONE) ? ANNUAL REPORT PURSUANT TO SECTION 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 FOR THE FISCAL YEAR ENDED DECEMBER 31, 2020 OR ? TRANSITION REPORT PURSUANT TO SECTION 1

June 14, 2021 8-K

Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): June 14, 2021 Enzo Biochem, Inc.

June 11, 2021 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2021 ☐ TRANSITION REPORT PUR

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q Mark one ? QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended April 30, 2021 or ? TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-09974 ENZO BIOCHEM

June 11, 2021 EX-99.1

Conference call and live webcast scheduled for today, Wednesday, June 9, 2021 at 4:30 PM (ET).

Exhibit 99.1 Enzo Biochem Reports Third Quarter Fiscal 2021 Results ? Total third quarter revenue of $32.8 million, a record quarterly high, increased 94% year-over-year ? Consolidated quarterly gross margin was 49% versus 26% in the previous year period, confirming Enzo?s vertically integrated approach can continue to drive healthy margins in a post pandemic environment ? Third consecutive quarte

June 11, 2021 8-K

Financial Statements and Exhibits, Results of Operations and Financial Condition

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): June 9, 2021 Enzo Biochem, Inc.

May 5, 2021 EX-99.1

Letter dated May 5, 2021.

Exhibit 99.1 ROUMELL ASSET MANAGEMENT, LLC 2 Wisconsin Circle, Suite 700 Chevy Chase, MD 20815 May 5, 2021 BY OVERNIGHT DELIVERY AND E-MAIL Enzo Biochem, Inc. 527 Madison Avenue New York, New York 10022 Attn: Ian B. Walters, M.D., member of the Board of Directors Mary Tagliaferri, M.D., member of the Board of Directors Rebecca J. Fischer, member of the Board of Directors Dov Perlysky, member of th

May 5, 2021 SC 13D/A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. 14) Enzo Biochem, Inc. (Name of Issuer) Common Stock (Title of Class of Securities) 294100102 (CUSIP Number)

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. 14) Enzo Biochem, Inc. (Name of Issuer) Common Stock (Title of Class of Securities) 294100102 (CUSIP Number) James C. Roumell Roumell Asset Management, LLC 2 Wisconsin Circle, Suite 700 Chevy Chase, MD 20815 (301) 656-8500 (Name, Address and Telephone Number

March 19, 2021 8-K

Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, DC 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of report (Date of earliest event reported): March 19, 2021 Enzo Biochem, Inc.

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